首頁> 外文期刊>Biochimica et Biophysica Acta. Molecular and cell biology of Lipids >Specific interaction between E2F1 and Sp1 regulates the expression of murine CTP:phosphocholine cytidylyltransferase alpha during the S phase.
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Specific interaction between E2F1 and Sp1 regulates the expression of murine CTP:phosphocholine cytidylyltransferase alpha during the S phase.

機(jī)譯：在S期，E2F1和Sp1之間的特異性相互作用調(diào)節(jié)了鼠CTP：磷酸膽堿細(xì)胞酰轉(zhuǎn)移酶α的表達(dá)。

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CTP:phosphocholine cytidylyltransferase alpha (CCTalpha) is a key enzyme for phosphatidylcholine biosynthesis in mammalian cells. This enzyme plays an essential role in all processes that require membrane biosynthesis such as cell proliferation and viability. Thus, CCTalpha activity and expression fluctuate during the cell cycle to achieve PtdCho requirements. We demonstrated, for the first time, that CCTalpha is localized in the nucleus in cells transiting the S phase, whereas it is localized in the cytoplasm of G(0)-arrested cells, suggesting a specific role of nuclear CCTalpha during the S phase. We also investigated how E2F1 influences the regulation of the CCTalpha-promoter during the S phase; we demonstrated that E2F1 is necessary, but not sufficient, to activate CCTalpha expression when this factor is over-expressed. However, when E2F1 and Sp1 were over-expressed, the transcription from the CCTalpha-promoter reporter construct was super-activated. Transient transfection studies demonstrated that E2F1 could super-activate Sp1-dependent transcription in a promoter containing only the Sp1 binding sites "B" or "C", and that Sp1 could activate Sp1-dependent transcription in a promoter containing the E2F site, thus, further demonstrating a functional interaction of these factors. In conclusion, the present results allowed us to portray the clearest picture of the CCTalpha-gene expression in proliferating cells, and understand the mechanism by which cells coordinate cell cycle progression with the requirement for phosphatidylcholine.
機(jī)譯：CTP：磷脂酰膽堿基轉(zhuǎn)移酶α（CCTalpha）是哺乳動(dòng)物細(xì)胞中磷脂酰膽堿生物合成的關(guān)鍵酶。該酶在需要膜生物合成的所有過程（例如細(xì)胞增殖和生存力）中起著至關(guān)重要的作用。因此，CCTalpha活性和表達(dá)在細(xì)胞周期中波動(dòng)，以達(dá)到PtdCho要求。我們第一次證明，CCTalpha定位在通過S期的細(xì)胞核中，而它定位在G（0）阻滯細(xì)胞的細(xì)胞質(zhì)中，表明SCC期間核CCTalpha的特定作用。我們還研究了E2F1在S期如何影響CCTalpha啟動(dòng)子的調(diào)控。我們證明了當(dāng)該因子過表達(dá)時(shí)，激活E2F1是必要的，但不足以激活CCTalpha表達(dá)。但是，當(dāng)E2F1和Sp1過表達(dá)時(shí)，CCTalpha啟動(dòng)子報(bào)告基因構(gòu)建體的轉(zhuǎn)錄被超激活。瞬時(shí)轉(zhuǎn)染研究表明，E2F1可以在僅包含Sp1結(jié)合位點(diǎn)“ B”或“ C”的啟動(dòng)子中超激活Sp1依賴性轉(zhuǎn)錄，而Sp1可以激活包含E2F位置的啟動(dòng)子中Sp1依賴性轉(zhuǎn)錄，因此，進(jìn)一步說明了這些因素的功能相互作用?？傊?，本研究結(jié)果使我們能夠描繪出增殖細(xì)胞中CCTalpha基因表達(dá)的最清晰圖片，并了解細(xì)胞協(xié)調(diào)細(xì)胞周期進(jìn)程與磷脂酰膽堿需求的機(jī)制。

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